Analysis of Flavonoid Contents in the Fruits of Acanthopanax Species using HPLC
2013
Lee, J.M., Chung-Ang University, Anseong, Republic of Korea | Lee, D.G., Chung-Ang University, Anseong, Republic of Korea | Lee, K.H., Chung-Ang University, Anseong, Republic of Korea | Choi, S.H., Gongju National University of Education, Gongju, Republic of Korea | Park, C.G., National Institute of Horticultural and Herbal Science, Eumseong, Republic of Korea | Lee, S.H., Chung-Ang University, Anseong, Republic of Korea
Analysis of flavonoid contents in the fruits of Acanthopanax species (A. chiisanensis, A. divaricatus, A. koreanum, A. senticosus, and A. sessiliflorus) was conducted by high performance liquid chromatography. A Discovery(R) C18 (4.6 × 250 mm, 5 μm) column was used with a gradient mobile phase of water and acetonitrile (90 : 10 to 60 : 40 for 60 min) and UV detection was conducted at 350 nm. The contents of rutin, hyperin, quercetin, afzelin, and kaempferol were 0.063~0.540, 0.494~7.480, 0.584~0.704, 0.388~0.567, 0.190~0.471 mg/g, respectively, in the fruits of Acanthopanax species. Total content of flavonoids in the fruits of Acanthopanax species was highest in those of A. chiisanensis. Furthermore, hyperin was the most abundant compound in the fruits of Acanthopanax species. Consequently, our results demonstrate that the fruits of Acanthopanax species containing flavonoids have promising potential as a new income source of agriculture and industry in medicinal natural products, health supplements, and beverages.
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