Reassessment of the Growth Properties of Mycelium among Cordyceps pruinosa Isolates According to Cultivation conditions
2013
Kim, J.Y., Dankuk University, Cheonan, Republic of Korea | Kwon, H.W., Dankuk University, Cheonan, Republic of Korea | Sung, G.H., RDA, Suwon, Republic of Korea | Choi, H.K., Chung-Ang University, Seoul, Republic of Korea | Sung, J.M., Mush Tech Inc., Kangwon, Republic of Korea | Kim, S.H., Dankuk University, Cheonan, Republic of Korea
To obtain basic data for the better use of Cordyceps pruinosa we reassessed the effect of different medium, culture method, pH, and carbon and nitrogen sources on the mycelial grwoth properties of four C. pruinosa isolates. The growth of mycelia differed among the four isolates depending on medium type and cultivation days. Among the tested 8 kinds of solid media, the four isolates grew well on PDA and MMMM. While , among the tested 8 kimds of liquid media, all the isolates grew well in SDYM The isolates also grew well in the SDYM with pH from 4.0 to 9.5 without any inhibition . One isolate could best grow at pH 8 to 9.5 . Regarding the ability of utilizing carbon source, the difference of meycelia growth among the isolates was the most with xylose. Regarding nitrogen source, three isolates could utilize urea which is new fact in this species. These results provide new points on the growth properties of the fungal mycelium which has not been explored before. Overall, this reassessed study concludied that it is necesary to check in advance the growth properties of mycelium when a new isolate of C. pruinosa is expected to be used for application.
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