Immunohistochemical localization of galectin-3 in the brain with Theiler's murine encephalomyelitis virus (DA strain) infection
2013
Shin, T., Jeju National University, Jeju, Republic of Korea | Carrillo-Salinas, F.J., Instituto Cajal, CSIC, Madrid, Spain | Mart'nez, A.F., Instituto Cajal, CSIC, Madrid, Spain | Mecha, M., Instituto Cajal, CSIC, Madrid, Spain | Guaz,a C., Instituto Cajal, CSIC, Madrid, Spain
Galectin-3 is a β-galactoside-binding lectin that plays a role in neuroinflammation through cell migration, proliferation, and apoptosis. In the present study, regulation of galectin-3 was examined in the brain of mice infected with the Daniel strain of Theiler’s murine encephalomyelitis virus (TMEV) at days 7 and 81 post-infection by immunohistochemistry. Immunohistochemistry revealed that galectin-3 was mainly localized in ionized calcium-binding adapter 1-positive macrophages/activated microglia, but not in Iba-1-positive ramified microglia. Galectin-3 was also weakly detected in some astrocytes in the same encephalitic lesions, but not in neurons and oligodendrocytes. Collectively, the present findings suggest that galectin-3, mainly produced by activated microglia/macrophages, may be involved in the pathogenesis of virus induced acute inflammation in the early stage as well as the chronic demyelinating lesions in Daniel strain of TMEV induced demyelination model.
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