Development and evaluation of protective capacity of Salmonella Enteritidis polyphosphate kinase-deleted and temperature-sensitive mutant
2013
Kim, K., Kangwon National University, Chuncheon, Republic of Korea | Park, S., Kangwon National University, Chuncheon, Republic of Korea | Cho, Y., Kangwon National University, Chuncheon, Republic of Korea | Kwak, J.Y., Kangwon National University, Chuncheon, Republic of Korea | Kang, Z.W., Kangwon National University, Chuncheon, Republic of Korea | Kim, E.H., ChoongAng Vaccine Lab., DaeJeon, Republic of Korea | Choi, H.W., ChoongAng Vaccine Lab., DaeJeon, Republic of Korea | Won, H.K., ChoongAng Vaccine Lab., DaeJeon, Republic of Korea | Noh, Y.H., ChoongAng Vaccine Lab., DaeJeon, Republic of Korea | Hahn, T.W.
This study was focusing on evaluating the protection of polyphosphate kinase (ppk) deleted and/or temperature-sensitive (ts) Salmonella Enteritidis (SE) as an attenuated vaccine in chickens. We constructed SEppk, SEts and SEppk::ts mutants and screened those mutants by growth capability in vitro, protection study in mice model and antibody response in chickens. Among the mutants, SEppk::ts-3 was selected because it showed higher growth capability, good protection against highly virulent SE in mice model, and good antibody response in chickens. SEppk::ts-3 also showed good protection against highly virulent SE isolate because it decreased colonization of virulent SE challenge strain in spleen, liver and cecum compared with the non-vaccinated control. The SEppk::ts-3 mutant showed crossprotection against S. Gallinarum (SG) challenge although the its cross-protection rate was a little lower than that of SG9R, a commercial vaccine against SG infection. To use for live attenuated vaccine in chickens, it should further be characterized.
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