Effects of Mustard Seeds and Power on In Vitro Ruminal Fermentation Characteristics and Methane Production
2013
Lee, K.Y., R.D.A., Suwon, Republic of Korea | Kim, K.H., R.D.A., Suwon, Republic of Korea | Back, Y.C., R.D.A., Suwon, Republic of Korea | Ok, J.U., R.D.A., Suwon, Republic of Korea | Seol, Y.J., R.D.A., Suwon, Republic of Korea | Han, K.J., R.D.A., Suwon, Republic of Korea | Park, K.K., Konkuk University, Seoul, Republic of Korea | Ryu, H.T., Nature and Technology Co.LTD, Sunchon, Republic of Korea | Lee, S.S., Sunchon National University, Sunchon, Republic of Korea | Jeon, C.O., Chung-Ang University, Seoul, Republic of Korea | Oh, Y.K., R.D.A., Suwon, Republic of Korea
The purpose of this study was to investigate the effects of mustard, which contains allyl isothiocyanate, on ruminal fermentation and methane emission in vitro. To this end, diluted ruminal fluid(30ml) was incubated anaerobically at 39℃ for 6, 12, and 24h with or without seeds or powdered mustard. Either mustard seed or powdered mustard was weighed and serially(0, 3.33, 5.00, 6.67, and 8.33g/L) mixed with ruminal fluid. Ammonia-N was increased(P0.05) by mustard treatment in a dose dependent manner. Regardless of concentration or form, mustard increased(P0.05) total VFA content but decreased(P0.01) pH compared to control group. Molar proportion of acetate(A) was decreased (P0.05) whereas propionate(P) was increased(P 0.05) by mustard treatment, thereby A:P ratio was decreased(P0.05) compared to control group. Total gas production was increased(P0.01) in a linear manner by mustard treatment compared to control group. There was no effect of mustard powder, except 8.33g/L level at 6h, on methane emission. However, at 24h, methane emission was reduced(P0.05) by 4.77% and 11.54% with 6.67g/L and 8.33g/L of mustard seeds supplementation, respectively. Altogether, these results suggest that mustard seeds containing allyl isothiocyanate may reduce methane production without disturbing ruminal fermentation.
显示更多 [+] 显示较少 [-]