Monitoring of Forbidden Medicines as Adulterants in Dietary Supplements Marketed Online by HPLC and ESI-tandom Mass Spectrometry
2012
Lee, Y.C., Seoul Metropolitan Government Research Institute of Public Health and Environment, Gwacheon, Republic of Korea | Kim, B.S., Seoul Metropolitan Government Research Institute of Public Health and Environment, Gwacheon, Republic of Korea | Yun, E.S., Seoul Metropolitan Government Research Institute of Public Health and Environment, Gwacheon, Republic of Korea | Kim, S.D., Seoul Metropolitan Government Research Institute of Public Health and Environment, Gwacheon, Republic of Korea | Chang, M.S., Seoul Metropolitan Government Research Institute of Public Health and Environment, Gwacheon, Republic of Korea | Park, Y.A., Seoul Metropolitan Government Research Institute of Public Health and Environment, Gwacheon, Republic of Korea | Shin, Y., Seoul Metropolitan Government Research Institute of Public Health and Environment, Gwacheon, Republic of Korea | Jung, S.O., Seoul Metropolitan Government Research Institute of Public Health and Environment, Gwacheon, Republic of Korea | Lee, J.I., Seoul Metropolitan Government Research Institute of Public Health and Environment, Gwacheon, Republic of Korea | Chae, Y.Z., Seoul Metropolitan Government Research Institute of Public Health and Environment, Gwacheon, Republic of Korea
It was carried out to detect anti-impotence drug-like compounds, sibutramine and their analogues in dietary supplements, which are doubtful whether they include illegal compounds. A total of 51 food products were bought online and have been investigated. The separation was achieved on a C18 column, with the mobile phase made up of water (5 mM sodium hexanesulfonate and 0.1% phosphoric acid) and 95% acetonitrile, at a flow rate of 1.2 mL/min with gradient elution using by HPLC-DAD. The UV signals were monitored at 220 nm and 291 nm. LC-ESI-tandom MS was utilized to confirm that detected compounds in samples are the same as the reference materials. Two nutrient supplement foods and ginseng products were found to contain 1.3-82.1 mg of sildenafil, dimethylthiolsildenafil and pseudovardenafil per serving size. In addition, two other processed products were detected to contain 1.7 and 2.2 mg of didesmethylsibutramine, derived from sibutramine per serving size.
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