Enhanced bacterial resistance in transgenic tobacco expressing a BrRZFP1 encoding a C3HC4-type RING zinc finger protein from Brassica rapa
2013
Jung, Y.J., Hankyong National University, Ansung, Republic of Korea | Nou, I.S., Sunchon National University,Suncheon, Republic of korea | Hong, S.K., National Academy of Agricultural Science, Suweon, Republic of Korea | Lee, Y.K., Crop Protection Division National Academy of Agricultural Science, Suweon, Republic of Korea | Cho, Y.G., Chungbuk National University, Cheongju, Republic of Korea | Kang, K.K., Hankyong National University, Ansung, Republic of Korea
C3HC4-type RING zinc finger proteins essential in the regulation of plant processes, including responses to abiotic stresses. We previously isolated and examined the C3HC4-type RING zinc finger protein (BrRZFP1) from Brassica rapa under abiotic stresses. To elucidate the role of the BrRZFP1 transcription factor in gene regulation, we transformed tobacco plants with the BrRZFP1 gene. Plants were regenerated from 82 independently transformed callus lines of tobacco and analysed for transgene expression. Transgene integration and expression was confirmed by Southern and RT-PCR analyses, respectively. T2 plants displayed more tolerance to the bacterial pathogens Pectobacterium carotovorum and Ralstonia solanacearum, and the tolerance levels were correlated with BrRZFP1 expression levels. These results suggest that the transcription factor BrRZFP1 is an important determinant of stress response in plants and its overexpression in plants could increase biotic stress resistance.
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