Plant regeneration through the callus culture induced from bulb scales of an endangered species Lilium cernum Komarvo.
2013
Bae, K.H., Kongju National University, Kongju, Republic of Korea | Yoon, H.S., Hongcheon Institute of Medicinal Herb, Hongcheon, Republic of korea
Lilium cernum Komarvo. is an important endangered plant belonging to the family Liliaceae. A method was developed for the rapid micropropagation of L. cernum through plant regeneration from bulb scales explant-derived calli. The bulb scales segments were cultured on Murashige and Skoog (MS) medium supplemented with 0, 0.5, 1.0, 3.0 mg․L-1 kinetin and 0, 0.1, 0.5, 1.0 mg․L-1 NAA or 2,4-D for callus induction. In media with 0.5∼3.0 mg․L-1 kinetin and 0.1∼1.0 mg․L-1 NAA and 2,4-D, 95∼100% of explants produced callus. They were then transferred to MS medium supplemented with various concentrations of NAA (0, 0.01, 0.05 and 1.0 mg․L-1) in combination with BA (0, 1.0 and 2.0 mg․L-1) for bulbet formation. Bulbet induction (78%), weight (468 mg) and size (15.5 mm) were obtained the highest on MS medium containing 2.0 mg․L-1 BA and 1.0 m g․L-1 NAA. In vitro frequency of plant regeneration was not significantly treated in strength of MS and sucrose concentration. Chlorophyll contents in 1/2MS with 50 g․L-1 sucrose treatments were higher than those in control and another treatment. This in vitro propagation protocol will be useful for conservation and mass propagation of this endangered plant.
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