Effects of Lycii Fructus and Lycii Folium Extracts on Osteoporosis in Ovariectomized Rats
2014
Kim, J.H., Donshin University, Naju, Republic of Korea | Kim, J.S., Donshin University, Naju, Republic of Korea
The effects of Lycii fructus and Lycii folium on osteoporosis and serum cholesterol levels were tested in ovariectomized (OVX) rats. Twenty-four female Sprague-Dawley rats were divided into four groups: Sham group (sham-operated), Control group (OVX, ovariectomized model), LCF group (Ovx+Lycii fructus extract), and LCL group (OVX+Lycium folium extract). After 8 weeks, the OVX ( 330¡¾5.39 g), LCF ( 315¡¾2.99 g), and LCL ( 318¡¾2.06 g) groups showed increased body weight compared with sham group ( 281¡¾1.71 g). The levels of serum osteocalcin (OC) also increased in the LCF ( 444.6¡¾26.9 ng/mL) and LCL ( 407¡¾18.9 ng/mL) groups compared with the OVX group ( 107¡¾3.52 ng/mL). The activities of serum alkaline phosphatase (ALP) increased in the LCF ( 108¡¾2.7 U/L) and LCL ( 407¡¾18.9 ng/mL) groups compared with the OVX group ( 95¡¾2.9 U/L). Stereomicroscopy found that the low bone density that developed in the OVX group was significantly reversed in the LCF and LCL groups after 8 weeks. We also obtained molecular-based in vivo evidence that supports a mechanism of action involving novel estrogen receptor ( ER¥� ) modulator in the uterus. We found that expression of ER ¥� mRNA in the OVX rat uterus was elevated by Lycium chinense. These results suggest that Lycii fructus and Lycii folium administered to rats during 8 weeks after oophorectomy may partially recover postmenopausal osteoporosis or delay the progression of osteoporotic changes.
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