Enzymatic Production of Amylopectin Cluster Using Cyclodextrin Glucanotransferase
2014
Lee, H.W., Hallym University, Chuncheon, Republic of Kroea | Jeon, H.Y., Hallym University, Chuncheon, Republic of Kroea | Choi, H.J., Hallym University, Chuncheon, Republic of Kroea | Shim J.H., Hallym University, Chuncheon, Republic of Kroea
To enzymatically prepare amylopectin cluster (APC), cyclodextrin glucanotransferase (CGTase I-5) and its mutant enzyme from alkalophilic Bacillus sp. I-5 were employed, after which the hydrolysis patterns of CGTase wild-type and its mutant enzyme toward amylopectin were investigated using multi-angle laser light scattering. CGTase wild-type dramatically reduced the molecular weight of waxy rice starch at the initial reaction, whereas the mutant enzyme degraded waxy rice starch relatively slowly. Based on the results, the molecular weight of one cluster of amylopectin could be about 104¢¦105 g/mol. To determine production of cyclic glucans from amylopectin, matrix-assisted laser desorption ionization time-of-flight mass spectrometry was performed. CGTase I-5 produced various types of cyclic maltooligosaccharides from amylopectin, whereas the mutant enzyme hardly produced any.
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