Evaluation of bacterial microflora of European eel (Anguilla anguilla) skin samples from lakes in Latvia
2014
Terentjeva, M., Latvia Univ. of Agriculture, Jelgava (Latvia). Faculty of Veterinary Medicine | Eizenberga, I., Latvia Univ. of Agriculture, Jelgava (Latvia). Faculty of Veterinary Medicine | Valcina, O., Institute of Food Safety, Animal Health and Environment „BIOR”, Riga (Latvia) | Novoslavskij, A., Lithuanian Univ. of Health Sciences, Kaunas (Lithuania). Faculty of Veterinary Medicine | Osmjana, J., Institute of Food Safety, Animal Health and Environment „BIOR”, Riga (Latvia) | Berzins, A., Latvia Univ. of Agriculture, Jelgava (Latvia). Faculty of Veterinary Medicine;Institute of Food Safety, Animal Health and Environment „BIOR”, Riga (Latvia)
Microflora of surfaces and skin of fish are continuously affected by aquatic environment including bacteria present in water, sediment and contamination from polluted wastewater. Above mentioned observations reveal that fish may serve as the indicator of pollution of aquatic environment, because the changes in microflora of surrounding environment of fish have an impact on number and composition of bacterial microflora of fish. Limited studies on bacterial contamination and the presence of pathogens in freshwater fish are conducted previously. Therefore the aim of the present study was to detect the total bacterial count (TBC) and Enterobacteriaceae on eel skin as indicator of hygienic status of environment and the presence of Salmonella spp. as possible zoonotic pathogenic agent of intestinal origin. For the detection of total bacterial contamination of eel the surface swabs were collected. Altogether 31 samples of eel skin were collected between April-May in 2014 from eels from three different lakes in Latvia (Alūksnes, Usmas and Sīvers). Skin samples were taken from live eel skin by processing a 10x10cm2 of skin with abrasive sponge. Skin samples were plated onto PCA and VRBA agar with subsequent incubation at 30°C and 37°C according to the ISO 6887 and ISO 21528 methods. For the detection of Salmonella the ISO 6579 method was applied. Depending on lake TBC of eel skin samples was varying from 5 up to 85000 cfu/cm2 , there the lowest average number of 36 cfu/cm2 was identified on eel skin originated from Alūksnes lake, and the highest average - 12750 cfu/cm2 in samples from Sīvers lake. Number of Enterobacteriaceae was varying from 0 up to 198 cfu/cm2 , there less than one cfu/cm2 was identified in samples from Alūksnes and Usmas lakes, and the highest average number - 36 cfu/cm2 in samples of Sīvers lake. All tested eel skin samples were Salmonella negative.
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