Isolation and functional characterization of the Brassica napus cruciferin gene cru4 promoter
2014
Roh, K.H., National Academy of Agricultural Science, RDA, Jeonju, Republic of Korea | Choi, S.B., National Academy of Agricultural Science, RDA, Jeonju, Republic of Korea | Kang, H.C., National Academy of Agricultural Science, RDA, Jeonju, Republic of Korea | Kim, J.B., National Academy of Agricultural Science, RDA, Jeonju, Republic of Korea | Kim, H.U., National Academy of Agricultural Science, RDA, Jeonju, Republic of Korea | Lee, K.R., National Academy of Agricultural Science, RDA, Jeonju, Republic of Korea | Kim, S.H., National Academy of Agricultural Science, RDA, Jeonju, Republic of Korea
The 12S globulin protein cruciferin is main seed storage protein in Brassica napus. To gain a better understanding of the Bncru4 promoter function, we conducted the promoter 5¡� deletion analysis in transgenic Arabidopsis. In the ¥�-glucuronidase (GUS) expression assay, Bncru4 promoter was strongly active in transgenic seeds. In addition, deletion of RY-elements (?236 bp region) dramatically decreased the promoter activity in seed embryos; however, the GUS expression could be observed in seed coat. Further deletion up to ?113 bp region (removed up to the CAAT and TATA box), GUS expression was completely abolished in all tissues. These results were consistent with that of the GUS activity in transgenic seeds. Therefore, we consider that RYelement is crucial to the seed-specific expression of Bncru4 promoter
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