Effect of epitope-CpG-DNA-liposome complex without carriers on vaccination of respiratory syncytial virus infection
2014
Park, B.K., Hallym University, Chuncheon, Republic of Korea | Kim, D., Hallym University, Chuncheon, Republic of Korea | Wu, G., Hallym University, Chuncheon, Republic of Korea | Choi, S.H., Hallym University, Chuncheon, Republic of Korea | Kim, D.S., Yonsei University, Seoul, Republic of Korea | Lee, Y., Chungbuk National University, Jeonju, Republic of Korea | Kwon, H.J., Hallym University, Chuncheon, Republic of Korea
Respiratory syncytial virus (RSV) is a common virus related to disease in the lung epithelium of young children and infants. However, RSV vaccine has not yet been developed. Thus it is difficult to develop a whole-RSV vaccine due to induction of Th2-type hyper-immune responses. To overcome this limitation, we used CpG-DNA encapsulated within liposome complex (Lipoplex(O)) as an adjuvant for the induction of a Th1-dominated humoral response in animal experiments. However, vaccination with a complex of UV-irradiated RSV and Lipoplex(O) had no effect against RSV infection. To improve the efficacy of the RSV vaccine, we performed peptide-based epitope screening and evaluated the efficacy of the vaccine using a complex of epitope and Lipoplex(O). Two efficient B-cell epitopes were identified in nine candidate epitopes from the RSV-F protein. The vaccination with a complex of RSV-F protein epitope (F7 and F9) and Lipoplex(O), induced a prophylactic effect on the RSVinfection based on lung histopathology and mucus clearance from the lungs. Thus, further studies on the effect of the peptide vaccine against infection by multiple RSV strains, may allow fine-tuning of a potential vaccine, and improvement of the vaccine program against RSV.
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