Detection of Heparin in the Salivary Gland and Midgut of Aedes togoi
2014
Ha, Y.R., Pohang University of Science and Technology, Pohang, Republic of Korea | Oh, S.R., Pohang University of Science and Technology, Pohang, Republic of Korea | Seo, E.S., Pohang University of Science and Technology, Pohang, Republic of Korea | Kim, B.H., Pohang University of Science and Technology, Pohang, Republic of Korea | Lee, D.K., Kosin University, Busan, Republic of Korea | Lee, S.J., Pohang University of Science and Technology, Pohang, Republic of Korea
Mosquitoes secrete saliva that contains biological substances, including anticoagulants that counteract a host is hemostatic response and prevent blood clotting during blood feeding. This study aimed to detect heparin, an anticoagulant in Aedes togoi using an immunohistochemical detection method, in the salivary canal, salivary gland, and midgut of male and female mosquitoes. Comparisons showed that female mosquitoes contained higher concentrations of heparin than male mosquitoes. On average, the level of heparin was higher in blood-fed female mosquitoes than in non-blood-fed female mosquitoes. Heparin concentrations were higher in the midgut than in the salivary gland. This indicates presence of heparin in tissues of A. togoi.
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