A Novel Recombinant BCG Vaccine Encoding Eimeria tenella Rhomboid and Chicken IL-2 Induces Protective Immunity Against Coccidiosis
2014
Wang, Q., Hebei Normal University of Science and Technology, Key Laboratory of Preventive Veterinary Medicine of Hebei Province, Qinhuangdao. China | Chen, L., Hebei Normal University of Science and Technology, Key Laboratory of Preventive Veterinary Medicine of Hebei Province, Qinhuangdao. China | Li, J., JiLin University, Changchun, China | Zheng, J., JiLin University, Changchun, China | Cai, N., Hebei Normal University of Science and Technology, Foreign language institute, Qinhuangdao, China | Gong, P., JiLin University, Changchun, China | Li, S., JiLin University, Changchun, China | Li, H., JiLin University, Changchun, China | Zhang, X., JiLin University, Changchun, China
A novel recombinant Bacille Calmette-Guerin (rBCG) vaccine co-expressed Eimeria tenella rhomboid and cytokine chicken IL-2 (chIL-2) was constructed, and its efficacy against E. tenella challenge was observed. The rhomboid gene of E. tenella and chIL-2 gene were subcloned into integrative expression vector pMV361, producing vaccines rBCG pMV361-rho and pMV361-rho-IL2. Animal experiment via intranasal and subcutaneous route in chickens was carried out to evaluate the immune efficacy of the vaccines. The results indicated that these rBCG vaccines could obviously alleviate cacal lesions and oocyst output. Intranasal immunization with pMV361-rho and pMV361-rho-IL2 elicited better protective immunity against E. tenella than subcutaneous immunization. Splenocytes from chickens immunized with either rBCG pMV361-rho and pMV361-rho-IL2 had increased CD4+ and CD8+ cell production. Our data indicate recombinant BCG is able to impart partial protection against E. tenella challenge and co-expression of cytokine with antigen was an effective strategy to improve vaccine immunity.
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