Physicochemical Properties of beta-Glucan from Acid Hydrolyzed Barley
2015
Lee, S.H., Chungbuk National University, Cheongju, Republic of Korea | Jang, G.Y., Chungbuk National University, Cheongju, Republic of Korea | Hwang, I.G., Department of Agrofood Resources, National Academy of Agricultural Science, Gyeonggi, Republic of Korea | Kim, H.Y., Department of Functional Crop, National Institute of Crop Science, Gyeongnam, Republic of Korea | Woo, K.S., Department of Functional Crop, National Institute of Crop Science, Gyeongnam, Republic of Korea | Kim, K.J., Division of Rice and Winter Cereal Crop, National Institute of Crop Science, Jeonbuk, Republic of Korea | Lee, M.J., Division of Rice and Winter Cereal Crop, National Institute of Crop Science, Jeonbuk, Republic of Korea | Kim, T.J., Chungbuk National University, Cheongju, Republic of Korea | Lee, J., Chungbuk National University, Cheongju, Republic of Korea | Jeong, H.S., Chungbuk National University, Cheongju, Republic of Korea
his study was performed to investigate changes in the content and purity, as well as physical characteristics of beta-glucan extracted from acid hydrolyzed whole grain barleys. Waxy and non-waxy barleys (Hordeum vulgare) were hydrolyzed with different concentrations of HCl (0.1~0.5 N) for 1 h. As the HCl concentration increased, the contents of total and soluble beta-glucan from acid hydrolyzed barley decreased. However the ratio of soluble/total beta-glucan content and purities of beta-glucan significantly increased. The ratio of Òbeta-(1 - 4)/ beta-(1 -3) linkages, molecular weight, and viscosity of soluble beta-glucan of raw barleys were 2.28~2.52, 6.0~7.0 x 105 g/mol, and 12.8~32.8 centipoise (cP). Those of isolated soluble beta-glucan were significantly decreased to 2.05~2.15, 6.6~7.8 x 103 g/mol, and 3.6~4.2 cP, respectively, with increasing acid concentration. The re-solubility of raw barley beta-glucan was about 50%, but increased to 97% with increasing acid concentration. Acid hydrolysis was shown to be an effective method to produce beta-glucan with high ratio of soluble beta-glucan content, purity, water solubility, and low viscosity.
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