Cell proliferation of silk proteins obtained from Bombyx mori silkworm varieties
2015
Chung, D.E., National Academy of Agricultural Science, RDA, Wanju, Republic of Korea | Kim, S.K., National Academy of Agricultural Science, RDA, Wanju, Republic of Korea | Jo, Y.Y., National Academy of Agricultural Science, RDA, Wanju, Republic of Korea | Kweon, H.Y., National Academy of Agricultural Science, RDA, Wanju, Republic of Korea | Lee, K.G., National Academy of Agricultural Science, RDA, Wanju, Republic of Korea | Kim, H., National Academy of Agricultural Science, RDA, Wanju, Republic of Korea
It is known that silk protein supports effectively proliferation of cell such as insect cell and hybridoma cell. Although there are many varieties of Bombyx mori silkworm, the effect of silkworm varieties on cell proliferation has not been considered in detail. We studied that characteristics of silk cocoon obtained from Baegokjam, Kumokjam, Daeseongjam silkworm varieties and whether silk protein affected cell proliferation or not. Silk sericin was prepared under high temperature and high pressure condition. Silk fibroin was prepared using CaCl2 : H2O : EtOH with different dissolution time. As a result, there are differences in silk cocoon from different silkworm varieties about cell proliferation. The proliferation was accelerated in the presence of Baegokjam silk sericin and Kumokjam silk fibroin with 5hr dissolution time. We expect that silk proteins could be a preferable culture medium supplement for stimulating the proliferation of cell. Then, this results suggest silk as a new material for medium supplement replacing with fetal bovine serum.
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