The effects of PEP-1-FK506BP on dry eye disease in a rat model
2015
Kim, D.W., Gangnung-Wonju National University, Gangneung, Republic of Korea | Lee, S.H., Lumieye Genetics Co., Ltd., Seoul, Republic of Korea | Ku, S.K., Daegu Haany University, Gyeongsan, Republic of Korea | Lee, J.E., Daegu Haany University, Gyeongsan, Republic of Korea | Cha, H.J., Hallym University, Chunchon, Republic of Korea | Youn, J.K., Hallym University, Chunchon, Republic of Korea | Kwon, H.Y., Hallym University, Chunchon, Republic of Korea | Park, J.H., Sookmyung Women;s University, Seoul, Republic of Korea | Park, E.Y., Sookmyung Women;s University, Seoul, Republic of Korea | Cho, S.W,, University of Ulsan College of Medicine, Seoul, Republic of Korea | Han, K.H., Hallym University, Chunchon, Republic of Korea | Park, J., Hallym University, Chunchon, Republic of Korea | Eum, W.S., Hallym University, Chunchon, Republic of Korea | Choi, S.Y., Hallym University, Chunchon, Republic of Korea
As FK506 binding proteins (FK506BPs) are known to play an important role in the regulation of a variety of biological processes related to cell survival, this study was designed to examined the protective effects of FK506 binding protein 12 (FK506BP) on low humidity air flow induced dry eye in a rat model using transduced PEP-1-FK506BP. After the topical application of PEP-1-FK506BP, tear volumes were markedly increased and significant prevention of cornea damage was observed compared with dry eye rats. Further, immunohistochemical analysis demonstrated that PEP-1-FK506BP markedly prevented damage to the cornea, the bulbar conjunctiva, and the palpebral conjunctiva epithelial lining compared with dry eye rats. In addition, caspase-3 and PARP expression levels were found to be decreased. These results demonstrated that topical application of PEP-1-FK506BP significantly ameliorates dry eye injury in an animal model. Thus, we suggest that PEP-1-FK506BP can be developed as a new ophthalmic drop to treat dry eye diseases.
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