Molecular Characterization of Trypanosoma cruzi Tc8.2 Gene Indicates Two Differential Locations for the Encoded Protein in Epimastigote and Trypomastigote Forms
2015
Kian, D., Universidade Estadual de Londrina, Londrina, Brazil | Lancheros, C.A.C, Universidade Estadual de Londrina, Londrina, Brazil | Damiani, I.A.C., Universidade Estadual de Londrina, Londrina, Brazil | Fernandes, T.Z.O., Universidade Federal de Sao Paulo, Sao Paulo, Brazil | Phileno, P.F, Universidade Estadual de Londrina, Londrina, Brazil | Machado dos Santos, M.R., Universidade Bandeirante de Sao Paulo, Sao Paulo, Brazil | Franco da Silveira, J., Universidade Federal de Sao Paulo, Sao Paulo, Brazil | Nakamura, C.V., Universidade Estadual de Maringa, Maringa, Brazil | Santana da Silva, J., Universidade de Sao Paulo, Ribeirao Preto, Brazil | Sueli Fumie, Y.O., Universidade Estadual de Londrina, Londrina, Brazil | Yamauchi, L.M., Universidade Estadual de Londrina, Londrina, Brazil
This report describes the molecular characterization of the Tc8.2 gene of Trypanosoma cruzi. Both the Tc8.2 gene and its encoded protein were analyzed by bioinformatics, while Northern blot and RT-PCR were used for the transcripts. Besides, immunolocalization of recombinant protein was done by immunofluorescence and electron microscopy. Analysis indicated the presence of a single copy of Tc8.2 in the T. cruzi genome and 2-different sized transcripts in epimastigotes/ amastigotes and trypomastigotes. Immunoblotting showed 70 and 80 kDa polypeptides in epimastigotes and trypomastigotes, respectively, and a differential pattern of immunolocalization. Overall, the results suggest that Tc8.2 is differentially expressed during the T. cruzi life cycle.
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