Production of Adventitious Root and Analysis of Effective Components from in vitro Culture of Astragalus membranaceus
2015
Hur, M., NIHHS, RDA, Eumseong, Republic of Korea | Lee, D.Y., NIHHS, RDA, Eumseong, Republic of Korea | Lee, J.W., NIHHS, RDA, Eumseong, Republic of Korea | An, T.J., NIHHS, RDA, Eumseong, Republic of Korea | Lee, J.H., NIHHS, RDA, Eumseong, Republic of Korea | Kim, Y.G., NIHHS, RDA, Eumseong, Republic of Korea | Cha, S.W., NIHHS, RDA, Eumseong, Republic of Korea | Um, Y., NIHHS, RDA, Eumseong, Republic of Korea
Background : A series of studies were conducted to optimize adventitious root induction in vitro from explants of Astragalus membranaceus using various nutrient media supplemented with plant hormones. Methods and Results : Levels of active components were analyzed from adventitious roots induced under different media conditions. Among the different media conditions, Murashige and Skoog medium supplemented with 1.0mg.litter minus 1 indole-3-butyric acid resulted in the greatest adventitious root induction rate. The amount of the major active component of the adventitious roots of Ama1, calycosin-7-O-beta-D-glucopyranoside was higher than that of other adventitious root samples. Conclusions : These results suggest that the adventitious roots of A. membranaceus could be used for the commercial production of medicines.
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