Detection of antibodies against Corynebacterium pseudotuberculosis using surface plasmon resonance
2011
Yeoh No Na | Ngajidin Mat Siman | Sharifah Aminah Syed Mohamad | Dzaraini Kamarun | Ramlan Mohamed | Radin Siti Fadzlina Hirzin
Conventional methods of detecting Corynebacterium pseudotuberculosis, a bacterium that causes caseous lymphadenitis (CLA) in sheeps and goats focused on several serodiagnostic tests such as ELISA, Western blotting and various inhibition and precipitation techniques. This paper described a Surface Plasmon Resonance (SPR) protocol for the direct detection of polyclonal
antibodies against Corynebacterium pseudotuberculosis with immobilisation of the antigen on unmodified transducer surface. The lower limit of detection was determined to be 2 μg mL-1 of immobilised antigen (Ag). Sufficient binding interaction was monitored on unmodified transducer; and saturation of the binding interaction was observed at 80 μg mL-1 of interacted antibody.
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