Biochemical and bacterial diversity changes in the rumen of cattle fed acacia [Samanea saman (Jacq.) Merr.] pod meal
2012
Angeles, A.A.
Biochemical and bacterial diversity changes in the rumen of rumen-cannulated and growing cattle fed with Acacia [Samanea saman (Jacq.) Merr.] pod meal (APM) were determined by in vitro and in vivo methods. Proximate analysis, paper chromatography (PC), thin-layer chromatography (TLC) and Folin-Dennis method were used to quantitatively and qualitatively determine nutrients and compounds present in APM fractions and other diet components. Rumen fluid cellulolytic, total gas, CO sub 2 and CH sub 4 production, pH, total volatile fatty acid (TVFA) and ammonia-N, microbial protein production and changes in bacterial diversity were determined after feeding the animals varying levels of APM. Growth performance of growing cattle was also determined. Proximate analyses nutrient levels were comparable to the concentrate mix. Qualitative profiling by (PC) showed predominantly hydrolyzable tannins with a concentration of 16.42%. In rumen-cannulated animals, cellulytic activity, gas production, rumen pH, TVFA, ammonia-N, microbial protein production were not statistically different among treatments. Cellolytic bacterial species S. ruminatiun and C. straminisolvens, and saccharolytic bacterium T. saccharophilium were affected by APM. In growing heifers, APM feeding resulted in poor ADG and FCR (P less than 0.01) highest in vitro cellulytic activity (P less than 0.01) and the lowest (P less than 0.01 gas production. Simple regression of average 30 and 60 d tannic acid intake (ATAI) with ADG ad FCR showed negative linear relationship. The current study suggests that feeding APM in growing cattle above 15% (DM requirement) will result to poor growth by altering some rumen characteristics and bacterial diversity.
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