Evaluation of sorghum (Sorghum bicolor) core collection for drought tolerance: Pollen fertility and mean performance of yield traits and its components at reproductive stage
2015
Sakhi, S. (Kohat Univ. of Science and Technology, Kohat (Pakistan). Dept. of Botany) | Shafiq-ur-Rehman (Kohat Univ. of Science and Technology, Kohat (Pakistan). Dept. of Botany) | Okuno, K. (University of Tsukuba (Japan). Lab. of Plant Genetics and Breeding Science, Graduate School of Life and Environmental Sciences) | Shahzad, A. (National Agricultural Research Centre, Islamabad (Pakistan). National Inst. for Genomics and Advanced Biotechnology) | Jamil, M. (Kohat Univ. of Science and Technology, Kohat (Pakistan). Dept. of Biotechnology)
Drought stress occurring during the early reproductive growth stage can cause considerable reduction in yield due to influence on plant reproductive components. The objectives of the present study were to evaluate the response of sorghum core collection (a diversity research set of 107 sorghum accession from Africa and Asia) under drought stress at reproductive stage. Accessions were subjected to drought stress in pots for 12 days period when booting leaf emerged. During drought stressed period no dry leaves were observed in non-stressed plants, whereas in drought stressed plants the average leaf drying score (LDS) was recorded from 5 to 85% among accessions. Pollen fertility (POFE) was measured by acetocarmine staining, which showed significant differences among accessions under drought stress. Different yield traits according to sorghum descriptor were recorded. Principle component analysis (PCA) revealed first 3-PCs explained 74.6% variation under drought stress, whereas in non-stressed conditions the variation was 69.3%. Considering different selection criteria including leaf drying score (LDS), pollen fertility (POFE) and drought susceptibility index (DSI) accessions 17, 23, 65 and 66 were found drought tolerant indicating lower LDS, high POFE and lowest DSI, whereas accessions 42, 81, 82 and 84 were drought sensitive. The results led to the conclusion that genetic variation for drought tolerance at reproductive stage present in this material can be used as a good source of variation in breeding program for drought tolerance.
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