The expression of visfatin in mouse ovary and its regulatory effect on IFN-gamma
2016
Song, H. (Huazhong Agricultural Univ., Wuhan (China). Coll. of Veterinary Medicine) | Qiu-xia, L. (Huazhong Agricultural Univ., Wuhan (China). Coll. of Veterinary Medicine) | Rui-an, L. (Huazhong Agricultural Univ., Wuhan (China). Coll. of Veterinary Medicine) | Xiao, K. | Ke-mei, P.
The expression of visfatin in ovary and the changes of IFN-gamma in peripheral blood were detected by the immunohistochemical method and the double antibody sandwich ELISA test method to investigate the distribution situation of visfatin in the ovary and its regulatory effect on the development of mouse ovarian follicles. Our research showed that visfatin positive cells were mainly located in the follicle cell layer of ovary in different developmental stages. The strongly positive staining were seen in the cells of the granular layer of growing follicle, the positive cells were numerous and compressed, and the cells of the granular layer of mature follicle also showed significantly positive reaction though their amount was fewer. Visfatin positive cells were rarely expressed and were weakly positive in oocytes, and it lacked in germinal epithelium, theca folliculi, follicular matrix and atretic follicle. Statistical results showed that the visfatin level of the control group was not significantly different from that of the protein group or the antibody group in the peripheral blood, while the protein group was significantly different from antibody group (P less than 0.05). The above results suggested that visfatin positive cells are widely expressed in the grained layer of ovarian follicle in different development stages, which shows its links with the development of follicular. The effect of visfatin on the level of IFN-gamma in periphery blood tips the role of visfatin in regulating the development of follicles.
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