Identification of a gram negative Rahnella aquatilis strain from Rana temporaria chensinensis David in China
2016
Xue, Y. (Northeast Forest Univ., Barbin (China). Coll. of Wildlife Resource) | Zhang, D. (Northeast Forest Univ., Barbin (China). Coll. of Wildlife Resource) | Chen, J. (Chinese Academy of Agricultural Sciences, Harbin (China). Harbin Veterinary Research Inst.) | Meng, Y. | Zhang, Y.
Rahnella aquatilis strain bf008 is a rare Gram negative bacterium isolated from one of the liver samples of Rana temporaria chensinensis David in China. Phenotypic characterizations of the isolate were done based on the colony morphology, microscopic observations and biochemical tests. The result of antimicrobial susceptibility test revealed that bf008 was resistant to fosfomycin and cefazolin, and intermediately susceptible to cefoperazone and cefoxitin. 16S rRNA of bf008 was sequenced and analyzed with those of R. aquatilis reference strains. The phylogenetic relationship based on 16S rRNA sequence showed bf008 has a close genetic relationship with 12 R. aquatilis reference strains. The present study will be helpful to further understand the significance of different microbial species of the Rana temporaria chensinensis David.
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