Distribution of the Respiratory Pathways in the Isolated Mitochondria from Etiolated Leaves of Winter Wheat and Rye after the Action of Low Temperature
2016
Borovik O.A. | Grabelnych O.I.
The effect of low temperature (2 °С, 7 days) on the content of soluble carbohydrates in the leaves and oxidative activity of isolated mitochondria from the etiolated plants of winter wheat (Triticum aestivum L.) and winter rye (Secale cereale L.) has been studied. This paper describes the effect of low temperature on the distribution of the respiratory pathways in the isolated mitochondria from etiolated leaves of winter wheat and rye that are different by resistance to cold. With using the different oxidation substrates (malate, malate + rotenone, succinate, NADH and NADPH), we identified changes in the oxidative activity of winter wheat and rye mitochondria. In this work, the dependence of the functioning of cyanide-insensitive oxidase and rotenone-insensitive NAD(P)H dehydrogenases in the isolated mitochondria of winter cereals from content of the soluble carbohydrates is discussed.
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