The results of rye double happloids (Secale sereale L.) development with use of in vitro anther technology

s presence could not be breeding inbred lines in generations, as when forced self-pollination grains were not tied for self-incompatibility.

英语. In a course of the reseacrh there was studied in vitro anther technology using for winter rye (Secale sereale L.) double hybrids development. AH stages of anther technology were fulfilled. The most comprehensible artificial mediums for calluses formation were established. Double haploids were developed only at the use of the anthers of tetraploid rye variety Belaya Vezha. The study of the developed double haploids by seed-set of ear and by 1000 grains weight was carried out. The low ear seed-set (22.4%) on average for all samples was registered: the average WOO grains weight was 16.2 g. Follow-up studies on the working out of ways to increase the efficiency of in vitro unther technology for rye are necessary. However, despite the low percentage yield fertile doubled haploid (5.6% of the planted calluses), managed to get by plants regenerated (doubled haploid) from the anthers of tetraploid rye varieties Belaya Vezha, as well as their seed progeny. When using tetraploid rye populations and further research was needed on methods and techniques to improve the efficiency of anther culture technology. Noteworthy was the idea of ​​using pre-forms of polyploidy of diploid rye before applying technology anther culture in vitro, since, as couldn be seen from the results of these studies, regenerated weakened completely perished when using colchicine. To create a homozygous inbred lines as the parental components of hybrid varieties was also necessary preliminary introduction of genes in a population of self-fertility or the use of existing heterotic hybrids of rye, which were already present these genes. Without self-fertility gene&#039