Influence of harvest time on the phenolic content of horseradish leaves
2017
Tomsone, L., Latvia Univ. of Agriculture, Jelgava (Latvia). Faculty of Food Technology. Dept. of Food Technology | Kruma, Z., Latvia Univ. of Agriculture, Jelgava (Latvia). Faculty of Food Technology. Dept. of Food Technology
A perennial herb horseradish (Armoracia rusticana L.) contains biologically active substances and cultivated in temperate regions of the world. The aim of the current research was to determine the content of phenolic compounds and antioxidant properties of horseradish leaves depending on the harvest time. For experiments horseradish leaves three years at six different times (during the period from May to October) were collected. Fresh plant material was extracted with ethanol using conventional extraction. For all extracts total phenolic content (TPC), total flavonoid content (TFC) and antioxidant activity (DPPH˙, ABTS˙ + scavenging activity, reducing power) were determined using a spectrophotometric methods. Results showed that content of phenolic compounds and antioxidant activity of horseradish leaves were significantly affected by harvest time. The highest TPC and ABTS˙ + scavenging activity was observed in the horseradish leaves collected in May, but the highest TFC and antioxidant activity (DPPH radical scavenging activity and reducing power) was observed in June. The content of phenolics compounds and antioxidant activity significantly decreased during post flowering stage and continues till October. Horseradish leaves contained considerable amount of phenolics compounds and natural antioxidants. In further experiments, use of horseradish leaves as natural antioxidants in different food matrixes should be studied.
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