Investigations on the resistance of porcine commensal E. coli isolates to beta lactams
2015
Urumova, Valentina (Trakia University, Stara Zagora (Bulgaria). Faculty of Veterinary Medicine, Department of Veterinary Microbiology, Infectious and Parasitic Diseases)
The aim of this study was to describe the prevalence of antibiotic resistance to betalactams and to evaluate two resistance genes bla TEM and bla OXA-1 in Escherichia coli isolates from faeces on six swine farms in the Republic of Bulgaria. A total of 186 E. coli isolates from 192 faecal swabs were tested by the disk diffusion method to determine resistance patterns to 11 antimicrobial agents. Resistance to beta-lactams was determined by disk diffusion method, E-test, micro-broth dilution method and PCR. About 40.3% of the E. coli isolates from swine were resistant to ampicillin. The highest resistance was observed in E.coli isolates from weaned pigs to ampicillin - 60.0% and to cephalotin - 45.5 %. The E. coli isolates resistant to beta-lactams were examined for the presence of bla TEM and bla OXA-1 genes. The most common bla gene identified was bla TEM, which was found in 92.0% of swine isolates.
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