Investigation of gene pool and genealogical links between sheep breeds of southern Russia by blood groups and DNA microsatellites
2015
Zinovieva, Natalia (All-Russian Research Institute of Animal Husbandry, Moscow (Russian Federation)) | Selionova, Marina (All-Russian Research Institute of Sheep and Goat, Stavropol (Russian Federation)) | Gladyr, Elena (All-Russian Research Institute of Animal Husbandry, Moscow (Russian Federation)) | Petrović, Milan P. (Institute for Animal Husbandry, Zemun -Belgrade (Serbia)) | Caro-Petrović, Violeta (Institute for Animal Husbandry, Belgrade-Zemun (Serbia)) | Ružić-Muslić, Dragana (Institute for Animal Husbandry, Belgrade-Zemun (Serbia)) | Petrović, Milan M. (Institute for Animal Husbandry, Belgrade-Zemun (Serbia))
To study the gene pool and the establishment of genealogical relationships between breeds of sheep of different directions productivity bred in Russia, were used two classes of genetic markers - blood and DNA microsatellites. The included sample sheep are fine-wool Merino breeds: Grozny (GR), Caucasian (CA), Manychskij merino (MM), the Soviet Merino (SM), Stavropol (ST) and coarse wool breeds: Edilbaevskaya (ED), Karakul (CR) and Romanov (RO). For the study of erythrocyte, were selected antigens (blood group) in 1159 samples from 11 breeding farms. For microsatellite DNA study - 598 from 10 breeding farms. Microsatellite analysis revealed that the most polymorphic were Stavropol breed sheep that have identified an average of 18.27 alleles per locus were relatively conservative Romanov breed sheep - 9.7 alleles per locus. The minimum genetic distances established between Grozny and Soviet Merino - 0.0569 (for microsatellites) and 0.0741 (blood groups - later in the same sequence). The rocks of the Stavropol – Grozny were 0.0861 and 0, 0810. Whereas Stavropol and Soviet Merino 0.0861 and 0.1094. Also relatively close between Grozny – Edilbaevskoy , Grozny - Karakul, Edilbaevskoy - Karakul: 0.1364 and 0.0851, respectively; 0.1620 and 0.1208; 0.1875 and 0.1192. The highest genetic distances were between Stavropol and Karakul - 0.2664 and 0.1804, as well as between the Romanov and all studied species - 0.2491 ... 0.3211 and 0.1734 ... 0.2235.
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