Mapping-by-sequencing enabled fast forward genetics in crops with complex genomes.
2017
Zhu QianHao | Wilson, I. | Llewellyn, D.
Forward genetics is an approach that applies mutants to identify genes responsible for a mutant phenotype, and is traditionally achieved using map-based cloning, a time-consuming multistep procedure, particularly for crops with complex genomes that usually lack high-quality genetic maps with high-density markers. Next-generation sequencing (NGS) technologies are now providing tools for the identification of an unprecedented number of genetic markers to overcome this limitation. In combination with bulk segregant analysis, NGS-based strategies are able to identify causal mutations in a single step that combines together marker identification and mutation mapping. Since the first report on gene identification using this mapping-by-sequencing (MBS) approach in Arabidopsis, MBS has been expanded to many other plant species to identify not only point mutations, but also deletion mutations thanks to the development and application of novel strategies. Here, we summarize the most recent progress on MBS-based fine-mapping and identification of causal mutations in plants, with a focus on crops with large and complex genomes.
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