Comparison of the Efficiency of Different DNA Extraction Methods on Livestock Feeds and Those Raw Materials
2016
Shin, K.S., National Institute of Agricultural Sciences, RDA, Jeonju, Republic of Korea | Woo, H.J., National Institute of Agricultural Sciences, RDA, Jeonju, Republic of Korea | Lim, M.H., National Institute of Agricultural Sciences, RDA, Jeonju, Republic of Korea | Lee, J.H., National Institute of Agricultural Sciences, RDA, Jeonju, Republic of Korea | Qin, Y., National Institute of Agricultural Sciences, RDA, Jeonju, Republic of Korea | Park, S.K., Kyungpook National University, Daegu, Republic of Korea | Kwon, T.R., National Institute of Agricultural Sciences, RDA, Jeonju, Republic of Korea | Cho, H.S., National Institute of Agricultural Sciences, RDA, Jeonju, Republic of Korea
Recently, in Korea various kinds of genetically modified (GM) crops have been imported and used as a raw material to manufacture foods and livestock feeds, but the different social concerns about the benefits and the potential risks of GM crops are being shown with a different reaction from the public. Thus a persistent management is required for the safe utilization of genetically modified organism (GMO). PCR analysis of transgene into crop is generally performed for the efficient post management of GMOs. The most important prerequisite for the application of nucleic acid detections is to decide the effective DNA-extraction methods. Particularly, in the case of processed feeds, the nucleic acids of which may be damaged by heating, high pressure, pH treatments, fermentation, etc. in processing, DNA must be extracted with high sensitivity from the samples to perform the PCR successfully. In this study, seven of DNA-extraction methods used commercially and non-commercially were compared with respect to the yields and quality of DNA extracted from livestock feeds and those crop materials. Amounts of genomic DNA obtained from the extraction methods varies according to feed configurations and crop materials. The DNA yield and uniformity of samples extracted with PG, CTAB, and QF method is greater than that obtained from other extraction methods. In the DNA integrity of the selected extraction methods, PCR analysis showed distinct amplifications and similar patterns in detecting crop endogenous genes and GMO genes. These results would be applicable for the selection of an adequate DNA-extraction method in extracting processed feeds and/or crop materials.
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