Isolation and Quantitative Analysis of Tricin from Ears of Phragmites communis
2017
Woo, H.S., Gyeongsang National University, Jinju, Republic of Korea | Lee, S.Y., Nakdonggang National Institute of Biological Resources, Sangju, Republic of Korea | Hwang, B.S., Nakdonggang National Institute of Biological Resources, Sangju, Republic of Korea | Jeong, S.C., Nakdonggang National Institute of Biological Resources, Sangju, Republic of Korea | Kim, D.W., Nakdonggang National Institute of Biological Resources, Sangju, Republic of Korea
The aim of this study was to investigate a validation method for determination of tricin in Phragmites communis ears. Tricin was isolated with chromatographic methods and used as the standard substances for quantitative analysis. The structural determination was characterized by comparing their NMR spectral data with values reported in the literature. For validation, the specificity, linearity, precision, accuracy, detection limits, and quantification limits of tricin was measured by HPLC. The results show that the specificity was satisfied with retention time and diode array detector (DAD) spectrum by analysis of tricin using HPLC. The limits of detection (LOD) for tricin was 0.84 mg/ml. Recovery of tricin was 98.80~108.22% with R.S.D values less than 2%. Intra-day and inter-day precisions of tricin in P. communis ears were 99.96~100.96% and 99.01~100.40%, respectively. Therefore, application of tricin was validated by an analytical method as major compound in P. communis ears.
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