Improvement of indirect enzyme-linked immunosorbent assay for detection of Japanese encephalitis virus antibodies in swine sera
2017
Yang, D.K., Viral Disease Division,Animal and Plant Quarantine Agency, Gimcheon, Republic of Korea | Kim, H.H., Viral Disease Division,Animal and Plant Quarantine Agency, Gimcheon, Republic of Korea | Jo, H.Y., Viral Disease Division,Animal and Plant Quarantine Agency, Gimcheon, Republic of Korea | Lee, S.H., Viral Disease Division,Animal and Plant Quarantine Agency, Gimcheon, Republic of Korea | Jang, S.H., R and D Center, MEDIAN Diagnostics, Chuncheon, Republic of Korea | Lee, S.O., R and D Center, MEDIAN Diagnostics, Chuncheon, Republic of Korea | Choi, S.S., Viral Disease Division,Animal and Plant Quarantine Agency, Gimcheon, Republic of Korea | Cho, I.S., Viral Disease Division,Animal and Plant Quarantine Agency, Gimcheon, Republic of Korea
Japanese encephalitis (JE) is an important zoonosis caused by the mosquito-transmitted JE virus (JEV), which is a causative agent of reproductive failure in pregnant sows. Detection of JEV antibodies in swine is performed by hemagglutination inhibition (HI), virus neutralization (VN), and the plaque reduction neutralization test (PRNT). The most stringent PRNT is the 90% endpoint PRNT (PRNT∧90). These conventional assays are difficult to carry out in diagnostic laboratories with insufficient instruments or cell culture systems. An alternative assay that is easily conducted and time efficient is required. In this study, we improved the indirect enzyme-linked immunosorbent assay (I-ELISA) with clarified antigen for the detection of JEV antibodies. The I-ELISA results obtained from 175 swine serum samples were compared with HI, VN, and PRNT∧90 results. The sensitivity of I-ELISA was 91.8%, 95.0%, and 94.7% compared with HI, VN, and PRNT∧90 results, respectively. The specificity of I-ELISA was 92.2%, 94.7%, and 94.7% compared with HI, VN, and PRNT∧90 results, respectively. Moreover, the I-ELISA results were significantly correlated with the HI (r = 0.93), VN (r = 0.95), and PRN∧T90 (r = 0.92) results. These results suggest that the improved I-ELISA is useful for serosurveillance of JEV in swine.
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