Rapid Identification of Methylglyoxal Trapping Constituents from Onion Peels by Pre-column Incubation Method
2017
Kim, J.H., Hanyang University, Ansan, Republic of Korea | Kim, M.I., Hanyang University, Ansan, Republic of Korea | Ahmed Shah Syed, Hanyang University, Ansan, Republic of Korea | Jung, K., CHA University, Sungnam, Republic of Korea | Kim, C.Y., Hanyang University, Ansan, Republic of Korea
The methylglyoxal (MGO) trapping constituents from onion (Allium cepa L.) peels were investigated using pre-column incubation of MGO and crude extract followed by HPLC analysis. The peak areas of MGO trapping compounds decreased, and their chemical structures were identified by HPLC-ESI/MS. Among major constituents in outer scale of onion, 2-(3,4-dihydroxybenzoyl)-2,4,6-trihydroxy-3(2H)-benzofuranone (2) was more effective MGO scavenger than quercetin (6) and its 4'-glucoside, spiraeoside (3). After 1 h incubation, compound 2 trapped over 90% MGO at a concentration of 0.5 mM under physiological conditions, but compounds 3 and 6 scavenged 45%, 16% MGO, respectively. HPLC-ESI/MS showed that compound 2 trapped two molecules of MGO to form a di-MGO adduct and compounds 3 and 6 captured one molecule of MGO to form mono-MGO adducts, and the positions 6 and 8 of the A ring of flavonoids were major active sites for trapping MGO.
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