Bovine adenovirus type 3 virions cannot be rescued in vivo after full-length viral genome transfection in the absence of detectable polypeptide IX
2017
Zhang, P., North-west A and F University, Yangling, China | Xue, Q., Chinese Institute of Veterinary Drug Controls, Beijing, China | Ma, J,, North-west A and F University, Yangling, China | Ren, J., North-west A and F University, Yangling, China | Xia, S., North-west A and F University, Yangling, China | Zhang, L., North-west A and F University, Yangling, China | Wang, W., North-west A and F University, Yangling, China | Suresh K. Tikoo, University of Saskatchewan Saskatoon, Saskatchewan, Canada | Enqi Du, North-west A and F University, Yangling, China
Bovine adenovirus type 3 (BAdV3) is being used in the development of potential vehicles for gene therapy and vectored vaccine. To that end, a more comprehensive description of BAdV3 biology is essential. In this study, we focused on the role of pIX in BAdV3 virion rescue after full-length BAdV3 genome transfection. Initially, pIX deletion or initiation codon mutation abolished the production of progeny virions, which suggested that pIX was essential for the rescue of BAdV3 containing a full-length genome. Moreover, through transfection of a panel of pIX mutant BAdV3 genomes, we observed that the conserved N-terminus and the putative leucine zipper element (PLZP) were essential for virion rescue, whereas the C-terminus following the coiled-coil domain was non-essential. In addition, swap of the PLZP element and its following region of BAdV3 pIX to corresponding domains of human adenovirus type 5 (HAdV5) did not affect virion production, whereas swap of the entire pIX abolished production of progeny virions. We suggest that failure of the full-length BAdV3 pIX swap might be due to species specificity of its N-terminus region before the PLZP element.
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