Phelligridin D from Phellinus baumii Reduces Boar Sperm Viability
2016
Yi, Y.J., Chonbuk National University, Iksan, Republic of Korea | Lee, I.K., Chonbuk National University, Iksan, Republic of Korea | Seo, D.W., Chonbuk National University, Iksan, Republic of Korea | Yun, B.S., Chonbuk National University, Iksan, Republic of Korea | Lee, S.M., Chonbuk National University, Iksan, Republic of Korea
Phelligridin D (Phe D) is a compound isolated from Phellinus baumii, which is known for various biological activities. In this study, the authors examined the effect of Phe D on boar spermatozoa for its potential application in assisted reproductive technology for mammals. Sperm motility and deubiquitinylating activity significantly decreased when boar spermatozoa were incubated with Phe D (greater than 0.5 μM). The fluorescence intensities of dead sperm, and reactive oxygen species production increased after sperm incubation in the presence of Phe D. Although Phe D is associated with antioxidant and free radical scavenging activity, sperm viability deteriorated after its addition. This could lead to fertilization failure, including that following artificial insemination or in vitro fertilization. Phe D might have other biological functions in spermatozoa, and therefore requires additional studies in the future.
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