Screening of Tissue Papers for Nematode Extraction for the Baermann Funnel Method
2016
Kang, H., Pusan National University, Miryang, Republic of Korea | Eun, G., Pusan National University, Miryang, Republic of Korea | Ha, J., Pusan National University, Miryang, Republic of Korea | Lee, J., Pusan National University, Miryang, Republic of Korea | Kim, D., Pusan National University, Miryang, Republic of Korea | Kim, Y., Pusan National University, Miryang, Republic of Korea | Choi, I., Pusan National University, Miryang, Republic of Korea
The Baermann funnel method requires approximately four Kimwipe tissues for research a nematode count under a stereo microscope. To select more efficient and economical nematode extraction paper for nematode extraction, 15 different kinds of tissue papers were tested and compared with Kimwipe tissues. Nematode species used in the extraction efficiency tests include juvenile (J2) of Heterodera sp., J2 of Meloidogyne sp., Pratylenchus sp., Rhabditis sp., Acrobeloides sp., Panagrolaimus sp., Poikilolaimus sp. and Diplogasterida. The extraction efficiency varied between 42.0 to 88.8%. Considering costs, extraction efficacy, and clarity, the Pulling Kitchen Towel (Monalisa Co., Korea) is the best tissue, with clarity A, isolation efficiency of 69.4% (not significantly different compared to Kimwipe 1 ply 88.8%), and ½ costs per isolation of Kimwipe 2 ply.
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