Composition and abundance of microbiota in the pharynx in patients with laryngeal carcinoma and vocal cord polyps
2017
Hongli Gong, Fudan University, Shanghai, China | Boyan Wang, Fudan University, Shanghai, China | Yi Shi, Fudan University Pudong Medical Center, Shanghai, China | Yong Shi, Fudan University, Shanghai, China | Xiyan Xiao, Fudan University, Shanghai, China | Pengyu Cao, Fudan University, Shanghai, China | Lei Tao, Fudan University, Shanghai, China | Yuezhu Wang, Chinese National Human Genome Sequencing Center, Shanghai, China | Liang Zhou, Fudan University, Shanghai, China
The pharynx is an important site of microbiota colonization, but the bacterial populations at this site have been relatively unexplored by culture-independent approaches. The aim of this study was to characterize the microbiota structure of the pharynx. Pyrosequencing of 16S rRNA gene libraries was used to characterize the pharyngeal microbiota using swab samples from 68 subjects with laryngeal cancer and 28 subjects with vocal cord polyps. Overall, the major phylum was Firmicutes, with Streptococcus as the predominant genus in the pharyngeal communities. Nine core operational taxonomic units detected from Streptococcus, Fusobacterium, Prevotella, Granulicatella, and Veillonella accounted for 21.3% of the total sequences detected. However, there was no difference in bacterial communities in the pharynx from patients with laryngeal cancer and vocal cord polyps. The relative abundance of Firmicutes was inversely correlated with Fusobacteria, Proteobacteria, Actinobacteria, and Bacteroidetes. The correlation was evident at the genus level, and the relative abundance of Streptococcus was inversely associated with Fusobacterium, Leptotrichia, Neisseria, Actinomyces, and Prevotella. This study presented a profile for the overall structure of the microbiota in pharyngeal swab samples. Inverse correlations were found between Streptococcus and other bacterial communities, suggesting that potential antagonism may exist among pharyngeal microbiota.
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