Construction of a genetic linkage map and QTL mapping of agronomic traits in Auricularia auricula-judae
2017
Li-Xin Lu, Jilin Agricultural University, Changchun, China | Fang-Jie Ya, Jilin Agricultural University, Changchun, China | Peng Wang, Jilin Agricultural University, Changchun, China | Ming Fang, Jilin Agricultural University, Changchun, China | You-Min Zhang, Jilin Agricultural University, Changchun, China | Wei-Tong Zhang, Jilin Agricultural University, Changchun, China | Xiang-Hui Kong, Jilin Agricultural University, Changchun, China | Jia Lu, Jilin Agricultural University, Changchun, China
Auricularia auricula-judae is a traditional edible fungus that is cultivated widely in China. In this study, a genetic linkage map for A. auricula-judae was constructed using a mapping population consisting of 138 monokaryons derived from a hybrid strain (A119-5). The monokaryotic parent strains A14-5 and A18-119 were derived from two cultivated varieties, A14 (Qihei No. 1) and A18 (Qihei No. 2), respectively. In total, 130 simple sequence repeat markers were mapped. These markers were developed using the whole genome sequence of A. auricula-judae and amplified in A14-5, A18- 119, and the mapping population. The map consisted of 11 linkage groups (LGs) spanning 854 cM, with an average interval length of 6.57 cM. A testcross population was derived from crossing between the monokaryon A184-57 (from the wild strain A184 as a tester strain) and the mapping population. Important agronomic trait-related QTLs, including mycelium growth rate on potato dextrose agar for the mapping population, mycelium growth rate on potato dextrose agar and sawdust for the testcross population, growth period (days from inoculation to fruiting body harvesting), and yield for the testcross population, were identified using the composite interval mapping method. Six mycelium growth raterelated QTLs were identified on LG1 and LG4, two growth period-related QTLs were identified on LG2, and three yieldrelated QTLs were identified on LG2 and LG6. The results showed no linkage relationship between mycelium growth rate and growth period. The present study provides a foundation for locating genes for important agronomic characteristics in A. auricula-judae in the future.
显示更多 [+] 显示较少 [-]