Disorder of Trafficking System of Plasma Membrane and Vacuole Antiporter Proteins Causes Hypersensitive Response to Salinity Stress in Arabidopsis Thaliana
2017
Kim, J.H., Dong-A University, Busan, Republic of Korea | Chen, C., Dong-A University, Busan, Republic of Korea | Yun, H.R., Dong-A University, Busan, Republic of Korea | Lee, Y.S., Dong-A University, Busan, Republic of Korea | Yi, Y.B., Dong-A University, Busan, Republic of Korea | Kim, T.Y., Asia Seed Company, Icheon, Republic of Korea | Kim, H.U., Sejong University, Seoul, Republic of Korea | Heo, J.B., Dong-A University, Busan, Republic of Korea
Rab GTPases play an important role in regulating intracellular vesicular trafficking in eukaryotic cells. Previously, we found that Oryza sativa rice Rab11 (OsRab11) is required for the regulation of vesicular trafficking from the transGolgi network (TGN) to the plasma membrane (PM) and/or vacuoles. To further elucidate the relationship between vesicular trafficking and abiotic and biotic stresses, we determined OsRab11 expression levels under several environmental stress conditions. OsRab11 expression was induced by pathogens, jasmonic acid (JA), and high salt treatment. Under high salt conditions, dominant negative OsRab11(S28N) mutant plants exhibited a hypersensitive phenotype similar to that of sos1- 1, whereas overexpressed-OsRab11 plants showed resistance to high salt stress. When the expression of vacuolar and PM Na+ /H+ antiporter genes such as AtNHX1, AtNHX2, and AtSOS1 was examined, there was no significant difference between the wild-type and OsRab11(S28N) mutant plants. However, PM trafficking of AtSOS1-green fluorescent protein (GFP) in 35S::AtSOS1-GFP sos1-1 plants was severely impaired by T7- OsRab11(S28N) expression. Similarly, vacuolar trafficking of AtNHX2-GFP was inhibited by T7-OsRab11 (S28N) expression. These results indicate that trafficking of PM and vacuolar antiporter proteins by OsRab11 is important for high salt stress resistance.
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