Evaluation of Biogas Production Performance and Dynamics of the Microbial Community in Different Straws
2017
Xue Li, Yanbian University, Yanji, China | Yan-Hua Liu, Yanbian University, Yanji, China | Xin Zhang, Yanbian University, Yanji, China | Chang-Ming Ge, Yanbian University, Yanji, China | Ren-Zhe Piao, Yanbian University, Yanji, China | Wei-Dong Wang, Heilongjiang August First Land Reclamation University, Daqing, China | Zong-Jun Cui, China Agriculture University, Beijing, China | Hong-Yan Zhao, Yanbian University, Yanji, China
The development and utilization of crop straw biogas resources can effectively alleviate the shortage of energy, environmental pollution, and other issues. This study performed a continuous batch test at 35 degree Celsius to assess the methane production potential and volatile organic acid contents using the modified Gompertz equation. Illumina MiSeq platform sequencing, which is a sequencing method based on sequencing-by-synthesis, was used to compare the archaeal community diversity, and denaturing gradient gel electrophoresis (DGGE) was used to analyze the bacterial community diversity in rice straw, dry maize straw, silage maize straw, and tobacco straw. The results showed that cumulative gas production values for silage maize straw, rice straw, dry maize straw, and tobacco straw were 4,870, 4,032.5, 3,907.5, and 3,628.3 ml/g ·VS , respectively, after 24 days. Maximum daily gas production values of silage maize straw and rice straw were 1,025 and 904.17 ml/g ·VS, respectively, followed by tobacco straw and dry maize straw. The methane content of all four kinds of straws was greater than 60%, particularly that of silage maize straw, which peaked at 67.3%. Biogas production from the four kinds of straw was in the order silage maize straw greater than rice straw greater than dry maize straw greater than tobacco straw, and the values were 1,166.7, 1,048.4, 890, and 637.4 ml/g ·VS, respectively. The microbial community analysis showed that metabolism was mainly carried out by acetateutilizing methanogens, and that Methanosarcina was the dominant archaeal genus in the four kinds of straw, and the DGGE bands belonged to the phyla Firmicutes, Bacteroidetes, and Chloroflexi. Silage maize is useful for biogas production because it contains four kinds of straw.
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