Antifungal-demelanizing properties and RAW264.7 macrophages stimulation of glucan sulfate from the mycelium of the mushroom Ganoderma lucidum
2017
Wan Abd Al Qadr Imad Wan-Mohtar, University of Malaya, Kuala Lumpur, Malaysia | Christina Viegelman, University of Strathclyde, Scotland, United Kingdom | Anita Klaus, University of Belgrade - Faculty of Agriculture, Belgrade, Serbia | Sarina Abdul Halim Lim, University of Malaya, Kuala Lumpur, Malaysia
Underutilized mycelium of Ganoderma lucidum BCCM 31549 has been a significant source of a glucan sulfate (GS) possessing therapeutic activities. GS have been evaluated for their antifungaldemelanizing properties and nitrite oxide production from stimulated RAW264.7 macrophages. GS exhibited antifungal activity against Aspergillus niger A60 with a minimum inhibitory concentration of 60 mg/mL and a minimum fungicidal concentration of 100 mg/mL. At 60 mg/mL (sublethal) and 30 mg/mL (subinhibitory) doses of GS, the mycelium of A. niger A60 was successfully demelanized with a conidiophore head and black pigment reduction. Additionally, GS successfully stimulated RAW264.7 macrophage cells at a concentration of 500 ㎍/mL to produce 0.45 μM of nitric oxide. The GSstimulated RAW264.7 macrophages were morphologically similar to those treated with lipopolysaccharide. The results highlight a novel bifunctional property of mycelial GS from G. lucidum.
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