Mass production of Diglyphus isaea (Hymenoptera:Eulophidae), a biological control agent of a Korean populationof potato leaf miner Liriomyza huidobrensis (Blanchard)(Diptera: Agromyzidae)
2017
Rameswor MAHARJAN, National Institute of Crop Science, Pyeongchang, Republic of Korea | Kwon, M., National Institute of Crop Science, Pyeongchang, Republic of Korea | Kim, J., National Institute of Crop Science, Pyeongchang, Republic of Korea | Jung, C., Andong National University, Andong, Republic of Korea
The potato leaf miner, Liriomyza huidobrensis (Blanchard), is an important pest ofpotato throughout the world, including Korea. A method was developed for massrearing the parasitoid Diglyphus isaea (Walker) using faba bean, Vicia faba L.(Fabaceae), as the host-plant and L. huidobrensis as the insect host. Faba bean plantswere planted in pots and maintained in a greenhouse for about 15 d. Pots were thenexposed to adult leaf miners in oviposition cages for 4 h. Plants containing latesecond to early third instar larvae were exposed to adult D. isaea in parasitoid cagesfor 24 h. The leaf area per pot after 15 d was 597.9 cm2, which produced 103.33larvae per pot. The number of adult parasitoids emerging per pot was 72.5; about41% of these were female. The daily cost of parasitoid production was USD20.95per 1000 individual parasitoids. The methodology developed for D. isaea could beused to rear other ectoparasitoids such as Hemiptarsenus spp. and Pnigalio sp. withdifferent insect hosts like L. trifolii. This is why this mass-rearing information isimportant for securing test insect materials for ecological and biological study ofLiriomyza species, and also for developing a biological control for Liriomyza speciesother than L. huidobrensis by mass production of associated natural enemies. We arefacing rapid agro-ecosystem changes including pest systems. Continuous monitoringof Liriomyza in solanaceous crops is needed.
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