Impact of the natural immunomodulating composition on systemic and local immune system of subclinical mastitis affected cows | Dabīgas imūnmodulējošas kompozīcijas ietekme uz sistēmisko un lokālo imūnsistēmu govīm ar subklīnisko mastītu
2017
Gulbe, G., Riga Stradins Univ. (Latvia). August Kirchenshtein Inst. of Microbiology and Virology | Valdovska, A., Latvia Univ. of Agriculture, Jelgava (Latvia). Faculty of Veterinary Medicine;Latvia Univ. of Agriculture, Jelgava (Latvia). Research Lab. of Biotechnology | Donina, S., Riga Stradins Univ. (Latvia). August Kirchenshtein Inst. of Microbiology and Virology | Saulite, V., Riga Stradins Univ. (Latvia). August Kirchenshtein Inst. of Microbiology and Virology | Jermolajevs, J., Riga Stradins Univ. (Latvia). August Kirchenshtein Inst. of Microbiology and Virology | Peskova, L., Riga Stradins Univ. (Latvia). August Kirchenshtein Inst. of Microbiology and Virology | Svirskis, S., Riga Stradins Univ. (Latvia). August Kirchenshtein Inst. of Microbiology and Virology
Subclinical mastitis is a specific condition of mammary gland characterized by prolonged low-intensity infection and subsequent tissue damages with a decrease in milk quality. One of the promising approaches in treatment of mastitis is immunomodulation, which promotes the elimination of causative agents and the recovery of homeostasis in the mammary gland. The aim of the study was to evaluate effect of composition LLG both on cow systemic and udder local immune system parameters. A total of 10 cows with subclinical mastitis received an intramammary infusion in each quarter of LGG which consist of lysozyme, lactic acid and glycopeptides isolated from L. helveticus (in 18 experimental udder quarters) or 0.9% NaCl (in 18 control udder quarters) 3 times with 48 h intervals. During the treatment number of lymphocytes and neutrophils significantly decreased in experimental cows’ blood while the same parameters increased in milk. CD8+ cells did not change remarkably, but number of CD4+, CD25+, CD38+, CD69+ and CD95+ cells was diminished in treated group during the study. 21 days after the last infusion of LLG rapid increasing of CD25+ cells was detected. Significant elevation of somatic cell count (SCC) in treated group was observed besides the number of pathogenic bacteria significantly decreased, and their amount on day 7 was close to zero. The composition LLG induces migration of lymphocytes and banded leukocytes from peripheral blood stream to mammary gland, causing a significant SCC increase and a decrease in number of pathogenic bacteria in milk. Furthermore LLG activates an acquired immunity.
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