Performance evaluation of tissue culture-derived cassava planting materials under field conditions
2017
Acedo, V.Z. | Arradaza, C.C.
Cassava (Manihot esulenta Crantz) is one of the most important root crops due to its many uses. The demand for cassava has increased drastically in recent years but production expansion is hindered by problems on lack of clean planting materials. Tissue culture techniques made possible the rapid propagation of NSIC approved cassava varieties. However, the tissue culture-derived materials are quite expensive, hence ex vitro techniques of mass propagation tissue cultured materials is needed. A two node propagation technique for clean tissue cultured plantlets was introduced earlier for rapid propagation without any promise on yield enhancement. This research was conducted to compare field performance of 1st generation tissue cultured plantlets (TC) with the two node (abt. 2 in) and 8 inches stem cuttings from second generation tissue culture-derived plants of the different cassava varieties namely CV 40, 39, 32, and 30. The materials were planted under field condition following the recommended cultural management practices. After 8 months from planting, for NSIC Cv39 and 40, the TC plants produced better root yield than the 2-node and 8 inches cuttings. In addition, healthier plants with longer stems having more nodes were observed in TC than the 2-node and 8 inches propagules. In NSIC Cv30 however, the TC plants had inferior performance in terms of root yield and plant height compared to the other two planting materials. In terms of average yield per plant, the 2-node cuttings in NSIC Cv32 had the highest root yield while the 8-inch cuttings produced the biggest stems.
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