Effect of myo-inositol supplementation on whole body proximate composition, fatty acid profile and antioxidant status of Nile tilapia (Oreochromis niloticus) fingerlings
2016
Hanif, M.
Present research was executed to determine the effect of graded levels of myo-inositol supplementation on whole body proximate composition, fatty acid profile, antioxidant status, thiobarbituric acid reactive substances (TBARS) assay and hepatopancreatic digestive enzymes activities in Nile tilapia (Oreochromis niloticus) fingerlings. The fingerlings were fed with five experimental diets namely D1, D2, D3, D4, and D5 containing myo-inositol at the level of 0, 100, 200, 400 and 800 mg/kg. Duplicate groups of fish were fed one of the diets once a day at 2% of its live weight for 2 months. Ten fishes from each replicate were sacrificed and their whole body and hepatopanceas were stored in refrigerator at -20 degree C temperature. One way analysis of variance (ANOVA) was used to analyze the resulting data. Whole body moisture content, crude fat and crude protein were significantly (p less than 0.05) increased while crude ash was significantly decreased in response to myo-inositol supplementation. Activities of antioxidant enzymes including superoxide dismutase (SOD) and catalase (CAT) were significantly increased in the whole body of Nile tilapia fed myo-inositol supplemented diet. TBARS content in the whole body and hepatopancreatic amylase, protease and lipase activities were significantly (p less than 0.05) increased in response to myo-inositol supplementation. Arachidonic acid and ARA/EPA ratio were significantly (p less than 0.05) increased while decosahexaenoic acid significantly (p greater than 0.05) decreased in fish fed myo-inositol supplemented diets. In conclusion, by increasing the concentration of myo-inositol, proximate composition, activities of digestive enzymes and antioxidant status were improved in Nile tilapia.
显示更多 [+] 显示较少 [-]