UV radiation mutagenesis of Pleurotus ostreatus for enhanced production of Laccase
2015
Siddique, A.
Lignocellulose is the major structural component of all plants and most abundant recalcitrant renewable material available in nature. A large volume of lignocellulosic wastes and residues wheat straw, rice straw, sugarcane, bagasse, com stover, banana stalk and com cobs produced in agricultural industries of Pakistan are not properly discarded and have become a major source of ecological pollution. Fungi are most commonly used organisms for lignocellulosic waste treatment in industry and biological pulping and bleaching. They decompose the lignocellulosic material of agro-waste in to smaller compounds with help of oxidative enzymes. Laccases are an interesting group of multi copper enzymes, which have exploited to oxidize phenolic, aromatic amines and lignocellulosic compounds. They are being investigated due to various biotechnological applications at industrial level such as biological delignification and pulp bleaching, removal of phenolic pollutants in soil, better treatment of wastewater, enzymatic decolorization of dye, processing of food, making of biosensors to estimate the amount of phenol in fruit juices and toxic compounds bioremediation in water. In this study Pleurotus ostreatus fungus was subjected to UV irradiation mutagenesis for hyper production of laccase enzyme. Different culture conditions like pH, temperature, fermentation time moisture level and inoculum size was optimized through Response Surface Methodology to attain the maximum yield of laccase.
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