In vitro effect of raw extract of Serbian genotype of garlic (Allium sativum l.) on eggs of gastrointestinal nematodes of sheep
2015
Simin, Stanislav (Faculty of Agriculture, Novi Sad (Serbia)) | Vlajić, Slobodan (Faculty of Agriculture, Novi Sad (Serbia)) | Simin, Vladislav (Faculty of Agriculture, Novi Sad (Serbia)) | Kuruca, Ljiljana (Faculty of Agriculture, Novi Sad (Serbia)) | Lalošević, Vesna (Faculty of Agriculture, Novi Sad (Serbia))
Negative impact of gastrointestinal nematodes (GIN) on the productivity of grazing sheep has long been recognized. In the majority of sheep-rearing countries, their control has long relied on the intensive use of broad-spectrum anthelmintics, which led to the widespread development of anthelmintic resistance. The use of herbal devormers, such as garlic, has been suggested as one of the alternative ways of controlling parasitism in producing animals. The aim of the present study was to investigate the in vitro effect of raw garlic extract (RGE), prepared from a Serbian genotype of the plant, on the hatching of sheep GIN eggs. For this purpose, an egg hatch test was performed using three different concentrations of garlic extract. Significant, dose-dependent, ovicidal effect of RGE (p=0.005) was recorded. Extract concentrations of 16.7%, 33.3% and 66.7% (v/v) inhibited hatching of 68.1%, 76.7% and 92.6% (median values) of eggs, respectively. Significantly higher efficacy was achieved with the highest concentration than with the lower ones (p˂0.05). In vivo confirmation of this effectiveness is necessary in order to evaluate practical use of extract under farming conditions.
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