Vegetative compatibility and rflp analysis of Colletotrichum destructivum isolates from alfalfa and red clover
2016
Vasić, Tanja (Institute for Forage Crops, Kruševac (Serbia)) | Krnjaja, Vesna (Institute for Animal Husbandry, Belgrade-Zemun (Serbia)) | Jevremović, Darko (Fruit Research Institute Čačak (Serbia)) | Stanković, Slavica (Maize Research Institute Zemun Polje, Belgrade-Zemun (Serbia)) | Terzić, Dragan (Institute for Forage Crops, Kruševac (Serbia)) | Milenković, Jasmina (Institute for Forage Crops, Kruševac (Serbia)) | Marković, Jordan (Institute for Forage Crops, Kruševac (Serbia))
A total of 17 isolates of Colletotrichum from alfalfa (Medicago sativa L.) and red clover (Trifolium pratense L.) plants with anthracnose symptoms were collected from 11 districts in Serbia during 2005-2010 and tested for variability in vegetative compatibility groups (VCGs) and restriction fragment length polymorphisms (RFLP). Nitrate nonutilising (nit) mutants were isolated from each of investigated C. destructivum isolates by selecting chlorate-resistant sectors on medium with chlorate. The isolates were grouped in five VCGs while one isolate was self-incompatible. No relationship was found between VCGs and geographical origin of the isolates. Restriction Fragment Length Polymorphism (RFLP) analysis of a 900 bp intron of the glutamine synthetase (GS) gene revealed a unique polymorphic profile of C. destructivum isolates, distinct from the profiles of other Colletotrichum species. An identical profile was produced for all C. destructivum isolates, regardless of their host and geographical origin. PCR-RFLP failed to detect some the Serbian C. destructivum isolates.
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